Antituberculosis nanodelivery system with controlled-release properties based on para-amino salicylate–zinc aluminum-layered double-hydroxide nanocomposites

Antituberculosis nanodelivery system with controlled-release properties based on para-amino salicylate–zinc aluminum-layered double-hydroxide nanocomposites

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Bullo Saifullah,1 Mohd Bocce Zobir Hussein,1 Samer Hasan Hussein-Al-Ali,2 Palanisamy Arulselvan,3 Sharida Fakurazi3,41Materials Synthesis and Characterization Laboratory, 2Laboratory of Molecular Biomedicine, 3Laboratory of Vaccines and Immunotherapeutics, 4Department of Human Anatomy, Universiti Putra Malaysia, Serdang, Selangor, MalaysiaAbstract: We report the intercalation and characterization of para-amino salicylic acid (PASA) into zinc/aluminum-layered double hydroxides (ZLDHs) by two methods, direct and indirect, to form nanocomposites: PASA nanocomposite prepared by a direct method (PASA-D) and PASA nanocomposite prepared by an indirect method (PASA-I).Powder X-ray diffraction, Fourier-transform infrared spectroscopy, and thermogravimetric analysis revealed that the PASA drugs were accommodated within the ZLDH interlayers.The anions of the drug were accommodated as an alternate monolayer (along the long-axis orientation) between ZLDH interlayers.Drug loading was estimated to be 22.8% and 16.

6% for PASA-D and PASA-I, respectively.The in vitro release properties of the drug were investigated in physiological simulated phosphate-buffered saline solution of pH 7.4 and 4.8.The release followed the pseudo-second-order model for both nanocomposites.

Cell viability (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide [MTT] assays) was assessed against normal human lung fibroblast MRC-5 and 3T3 mouse fibroblast cells at 24, 48, Craft Apron and 72 hours.The results showed that the nanocomposite formulations did not possess any cytotoxicity, at least up to 72 hours.Keywords: drug-delivery system, slow-release nanocarrier, tuberculosis, biocompatible nanocomposites.